Inhibition of TLR4 Signalling-Induced Inflammation Attenuates Secondary Injury after Diffuse Axonal Injury in Rats

Figure 2

Dynamic expression of proteins related to the TLR4 signalling pathway after DAI. (a) Western blotting analysis was performed to measure the dynamic expression of TLR4, MyD88, TRIF, IRF3, and IFN-β at 12 h, 1 d, and 3 d after DAI. (b) The bar graphs show the cortical expression results for proteins related to the TLR4 signalling pathway. The expression of β-actin was used as an internal control. (c) Western blotting analysis was performed to measure the dynamic expression of NF-κB and phospho-NF-κB expression in nuclear extract at 12 h, 1 d, and 3 d after DAI. (d) The bar graphs show the results for NF-κB and phospho-NF-κB expression in the nuclear extract. The expression of lamin B was used as an internal control. Values are presented as the mean ± SD (; , compared with control group; , compared with DAI 1 d group).

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