Inhibition of TLR4 Signalling-Induced Inflammation Attenuates Secondary Injury after Diffuse Axonal Injury in Rats

Figure 3

Localization of TLR4 in different cell types after DAI. (a–c) Double immunofluorescence staining was performed with an antibody to TLR2 and antibodies to GFAP (marker for astrocytes), NeuN (marker for neurons), and Iba-1 (marker for microglia). Antibody binding was demonstrated using the following fluorophore-conjugated secondary antibodies: TLR4 (red), NeuN (green), GFAP (green), and Iba1 (green). Nuclei were stained with DAPI (blue). Scale bar = 50 μm. (d) The bar graphs show the results for the numbers of TLR4- and NeuN/GFAP/Iba-1-positive cells in the control and DAI 1 d groups. Values are presented as the mean ± SD (; ).

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