Inhibition of TLR4 Signalling-Induced Inflammation Attenuates Secondary Injury after Diffuse Axonal Injury in Rats

Figure 8

TLR4 inhibition attenuated glial responses. (a) The immunoreactivity of the astrocyte marker GFAP and that of the microglial cell marker Iba-1 were assessed via immunohistochemistry in rat cortices in the control, DAI 1 d, DAI 1 d+vehicle, and DAI 1 d+TAK-242 groups. Scale bar = 50 μm. (b) The bar graphs show the results for GFAP and Iba-1 immunoreactivity, as determined via immunohistochemistry. GFAP and Iba-1 levels were assessed in 5 random fields (40x magnification) by immunohistochemical scores (IHS). (c) GFAP and Iba-1 expression was measured via western blotting. (d) The bar graphs show the results for GFAP and Iba-1 expression, as determined via western blotting. Data are presented as the mean ± SD (; , compared with control group; , , compared with DAI 1 d+vehicle group).

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