Induction of Mast Cell Accumulation by Tryptase via a Protease Activated Receptor-2 and ICAM-1 Dependent Mechanism

Figure 4

Inhibition of mast cell accumulation by antibodies (Ab) against cell adhesion molecules. Mice were pretreated with monoclonal Ab against L-selectin (anti-CD62L), CD11a (anti-CD11a), CD18 (anti-CD18), and ICAM-1 (anti-ICAM-1), respectively, for 30 min before intraperitoneal injection of (a) compound 48/80 (Comp, μg), lTryptase (lTrypt, μg), or trypsin (Tryps, μg); (b) SLIGRL-NH₂ (SLIGRL, μM) and tc-LIGRLO-NH₂ (tc-LIGRLO, μM) for 6 h. Normal saline (NS) was employed as carrier. Data were displayed as a boxplot, which indicates the median, interquartile range and the largest and smallest values. Each piece of data represented a group of 6-7 animals. compared with the corresponding NS group. compared with the corresponding stimulus alone group.

(a)

(b)