Induction of Mast Cell Accumulation by Tryptase via a Protease Activated Receptor-2 and ICAM-1 Dependent Mechanism

Table 1

Inhibition of tryptase induced mast cell infiltration by inhibitors of tryptase and heat inactivation of the enzyme at 6 and 16 h following injection.

Treatment	Percentage inhibition of mast cell infiltration
	6 h	16 h
	Median (range)	Median (range)
Leupeptin 10 g	91.2 (76.9–100)	81.1 (57.9–100)
Benzamidine 10 g	84.6 (71.8–100)	71.2 (54.8–100)
Aprotinin 10 g	82.3 (56.4–100)	87.4 (57.9–100)
Antipain 10 g	79.5 (61.5–100)	90.1 (62.3–100)
Protamine 10 g	81.2 (67.7–100)	NA
Heat inactivation 5 g	90.3 (81.2–100)	91.1 (81.3–100)

The values shown are median (range) for 6–8 individual mice. The inhibitors were incubated with 5 g of human lung tryptase for 20 min before injection into mouse peritoneum. compared with the uninhibited control mice. NA = not available.