Smac127 Has Proapoptotic and Anti-Inflammatory Effects on Rheumatoid Arthritis Fibroblast-Like Synoviocytes

<div>Smac127 induces caspase 3 activation. Western blot analysis shows the effect of Smac127 on caspase 3 in extracts from human fibroblast-like synoviocytes (FLSs) derived from patients with rheumatoid arthritis cultured in TM (a) or in SF for 5 days (b). Levels of cleaved caspase 3 were measured in RA-FLSs untreated (CTRL) or treated with Smac127 (15 <i>μ</i>M) or treated with the apoptosis inducer staurosporine (stauro). Cells cultured in tissue medium alone were incubated for 6 hrs, whereas cells grown in the presence of SF were incubated for 18 hrs; staurosporine 20 <i>μ</i>M was the positive control.</div>

Mediators of Inflammation

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Figure 5

Figure 5: Smac127 Has Proapoptotic and Anti-Inflammatory Effects on Rheumatoid Arthritis Fibroblast-Like Synoviocytes 