Protective Effect of Adrenomedullin on Rat Leydig Cells from Lipopolysaccharide-Induced Inflammation and Apoptosis via the PI3K/Akt Signaling Pathway ADM on Rat Leydig Cells from Inflammation and Apoptosis

Figure 2

Effect of ADM on the concentrations of ROS, MDA, LDH, GSH, and testosterone and the MMP in the primary culture of Leydig cells. Leydig cells were treated with ADM (100 nM) or ADM (100 nM) + LY294002 (10 μmol/L) for 2 h prior to a 12-hour treatment with LPS (1 μg/mL). TMRM fluorescence intensity was analyzed on behalf of MMP. Data were obtained from five independent experiments performed in triplicate and expressed as mean ± SEM.

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