Activation of Melanocortin Receptors MC₁ and MC₅ Attenuates Retinal Damage in Experimental Diabetic Retinopathy

Figure 2

Real Time PCR for the expression of melanocortin receptor subtypes within the retina. (a) Representative traces of the RT-PCR and (b) relative gene expressions for MC₁, MC₃, and MC₅ receptors assayed after 16-week follow-up in nondiabetic mice, and diabetic mice with retinopathy. Total RNA was extracted using RNeasy Plus Mini Kit and commercially available primer for amplification of mouse MC₁, MC₃, and MC₅ receptors. Negative controls were either RT without enzyme or PCR without cDNA template.

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