Interferon-Stimulated Gene 15 Conjugation Stimulates Hepatitis B Virus Production Independent of Type I Interferon Signaling Pathway In Vitro

Figure 3

HBV protein expression after ISG15 overexpression. HepG2.2.15 cells were transfected with the ISG15 plasmid or GFP-expressing plasmid. (a) HBsAg and (b) HBeAg in the culture medium were detected by ELISA, respectively. (c) Intracellular HBcAg was assessed by western blot. Relative integrated density was calculated by ImageJ software and normalized by GAPDH expression. PEI, transfection regent polyethyleneimine (PEI) treatment only; GFP, transfected with 4 μg GFP-expressing plasmid; ISG15, transfected with 4 μg ISG15 plasmid. The results are presented as the means ± SD, ; error bars indicate SD.

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