Interferon-Stimulated Gene 15 Conjugation Stimulates Hepatitis B Virus Production Independent of Type I Interferon Signaling Pathway In Vitro

Table 1

Primers used for real-time PCR.

	Nucleotide sequence
ISG15	F: 5′-CGCAGATCACCCAGAAGATT-3′
	R: 5′-GCCCTTGTTATTCCTCACCA-3′
GAPDH	F: 5′-GCCTCCTGCACCACCAACTG-3′
	R: 5′-ACGCCTGCTTCACCACCTTC-3′
HBV	F: 5′-CGTTTTTGCCTTCTGACTTCTTTC-3′
	R: 5′-ATAGGATAGGGGCATTTGGTGGTC-3′
HBV pgRNA	F: 5′-CTCAATCTCGGGAATCTCAATGT-3′
	R: 5′-TGGATAAAACCTAGCAGGCATAAT-3′
MxA	F: 5′-GTGCATTGCAGAAGGTCAGA-3′
	R: 5′-CTGGTGATAGGCCATCAGGT-3′
OAS3	F: 5′-GAATTCTCCCATCAAAGTGATCAA-3′
	R: 5′-CTCAGATGCCGACCTCGTGGT-3′
USP18	F: 5′- CAGACCCTGACAATCCACCT-3′
	R: 5′- AGCTCATACTGCCCTCCAGA-3′
IFN α	F: 5′-TCGCCCTTTGCTTTACTGAT-3′
	R: 5′-GGGTCTCAGGGAGATCACAG-3′
IFN β	F: 5′-AAACTC ATAGCAGTCTGCA-3′
	R: 5′-AGGAGATCTTCAGTTTCGGAGG-3′

pgRNA, pregenomic RNA; MxA, myxovirus resistance 1; OAS3, 2′-5′-oligoadenylate synthetase 3; USP18, Ubiquitin specific protease 18.