miR-155 Regulated Inflammation Response by the SOCS1-STAT3-PDCD4 Axis in Atherogenesis

Figure 3

The relative expression of PDCD4 in macrophages RAW264.7 cells. (a and b) Western blot and qPCR analyzed the PDCD4 protein and mRNA expression after indicated ox-LDL treated macrophages RAW264.7 cells for 24 h. Representative bands show the protein expression of PDCD4 (the upper panel), β-actin (the middle panel), and the histograms showing quantification of PDCD4 bands normalization relative to β-actin expression in (a): versus control, versus 5 μg/mL group, and versus 10 μg/mL group. (c and d) Western blot and qPCR analyzed the PDCD4 protein and mRNA expression while RAW264.7 cells were treated by 20 μg/mL ox-LDL plus anti-miR-155 or anti-NC. 100 pM anti-miR-155 or anti-NC had been transfected into RAW264.7 cells for 24 h, and then they were treated by 20 μg/mL ox-LDL for 24 h. Representative bands show the protein expression of PDCD4 (the upper panel), β-actin (the middle panel), and the histograms showing quantification of PDCD4 bands normalized relative to β-actin expression in (c): versus control, versus 20 μg/mL ox-LDL group, and versus 20 μg/mL ox-LDL plus anti-NC group.

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