miR-155 Regulated Inflammation Response by the SOCS1-STAT3-PDCD4 Axis in Atherogenesis

Figure 4

Downregulation of PDCD4 and miR-155 regulated the expression of IL-6, TNF-α, and IL-10. (a) Knocking down the expression of PDCD4 mRNA by PDCD4 siRNA in macrophages RAW264.7 cells. The relative expression of PDCD4 mRNA was detected by qPCR after 50pM PDCD4 siRNA, including si-01, si-02, and si-03, and negative control (NC) was transfected into RAW264.7 cells for 48 h, respectively: versus control, versus NC group. (b) Western blot analyzed the protein level of PDCD4 when RAW264.7 cells had been treated by 50pM PDCD4 siRNA (si-02) and NC for 48 h. Representative bands show the protein expression of PDCD4 (the upper bands), β-actin (the lower bands) in left panel, and the histograms in right panel showing quantification of PDCD4 bands normalization relative to β-actin expression in (b): versus NC group. (c, d, and e) qPCR analyzed the mRNA expression of TNF-α, IL-6, and IL-10 in RAW264.7 cells. They were transfected into the anti-miR-155 or PDCD4 siRNA for 24 h and then treated by ox-LDL for 24 h: versus control, versus 20 μg/mL ox-LDL group, and versus 20 μg/mL ox-LDL plus PDCD4 siRNA group. The data are presented as the mean ± SE of three separate experiments.

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