miR-155 Regulated Inflammation Response by the SOCS1-STAT3-PDCD4 Axis in Atherogenesis

Figure 5

miR-155 regulated directly SOCS1-STAT3 signal pathway. (a) Schematic of the interaction sites of 3′ UTR SOCS1 wild-type (WT) or mutation (Mut) with miR-155. (b) Luciferase reporter assay was used to validate miR-155 binding SOCS1 3′UTR in HEK293FT cells. The luciferase reporter plasmids carrying the WT or Mut 3′UTRof SOCS1 and miR-155 mimic/miR-negative control (NC) were cotransfected into HEK 293FT cells for 24 h, and then luciferase activity was detected: , relative to miR-NC group. (c) and (d) Western blot analyzed the protein expression of SOCS1 and p-STAT3 after miR-155 mimic, anti-miR-155, and miR-NC was transfected into RAW264.7 cells for 48 h, respectively: , relative to miR-NC group. The data are presented as the mean ± SE of three separate experiments.

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