miR-155 Regulated Inflammation Response by the SOCS1-STAT3-PDCD4 Axis in Atherogenesis

Figure 7

Inhibition of miR-155 by Anti-miR-155 decreased the atherosclerotic development in ApoE^−/− mice with HFD (ApoE^−/− HFD mice). (a) qPCR detected the miR-155 and CD68 mRNA expression in aortic tissue when ApoE^−/− HFD mice were injected with anti-miR-155 and anti-NC. versus anti-NC. (b) Atherosclerotic plaques in the thoracoabdominal aorta of anti-miR-155 and anti-NC-injected ApoE^−/− HFD mice were assessed by Oil Red O staining, and the plaques areas were quantified by using Imagepro-plus 7.0: versus anti-NC. (c) Western blot analyzed the protein level of SOCS1, p-STAT3, and PDCD4 in aortic tissue when ApoE^−/− HFD mice were injected with anti-miR-155 and anti-NC for 2 weeks. Representative bands show the protein expression of SOCS1 (the upper bands), p-STAT3 (the middle bands), PDCD4 (the third bands), and β-actin (the lower bands) in the left panel, and the histograms in the right panel show quantification of the bands’ normalization relative to β-actin expression in (c). The data are presented as the mean ± SE of three separate experiments: versus anti-NC. (d) The plasma level of IL-6, TNF-α, and IL-10 of anti-miR-155 and anti-NC-injected ApoE^−/− HFD was detected by the ELISA kit (): versus ApoE^−/− HFD group, versus anti-NC-injected ApoE^−/− HFD group. (e) qPCR analyzed the expression of IL-6, TNF-α, and IL-10 of c57 mice, ApoE^−/− ND, ApoE^−/− HFD, anti-miR-155, and anti-NC-injected ApoE^−/− HFD mice in aortic tissue (): , relative to c57 mice WT, , relative to ApoE^−/− ND, , relative to ApoE^−/− HFD.

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