Hydroxysafflor Yellow A Inhibits LPS-Induced NLRP3 Inflammasome Activation via Binding to Xanthine Oxidase in Mouse RAW264.7 Macrophages

Figure 7

HSYA inhibits NLRP3 inflammasome activation. RAW264.7 cells were pretreated with HSYA at indicated doses (25, 50, and 100 μM) for 3 h and then treated with 1 μg/mL LPS for 8 h. The GAPDH, NLRP3, ASC, procaspase-1, and caspase-1 proteins expressions were analyzed by Western Blotting. Data represent the mean ± SEM of three independent experiments and differences between mean values were assessed by one-way ANOVA. , , , , , and indicate significant differences compared with the control group of indicated proteins, respectively.