Hydroxysafflor Yellow A Inhibits LPS-Induced NLRP3 Inflammasome Activation via Binding to Xanthine Oxidase in Mouse RAW264.7 Macrophages

Figure 8

HSYA inhibits LPS-induced IL-1β and IL-18 secretion. RAW264.7 cells were pretreated with HSYA at indicated doses (25, 50, and 100 μM) for 3 h and then treated with 1 μg/mL LPS for 12 h (Western Blot) or 24 h (ELISA). (a) The pro-IL-1β, IL-1β, and GAPDH proteins expressions were analyzed by Western Blotting. (b) The IL-1β and IL-18 secretion were measured by ELISA. Data represent the mean ± SEM of three independent experiments and differences between mean values were assessed by one-way ANOVA. , , , and indicate significant differences compared with the control group of indicated proteins, respectively.

(a)

(b)