Uncoupling Protein 2 Increases Susceptibility to Lipopolysaccharide-Induced Acute Lung Injury in Mice

Figure 5

UCP2-induced inflammation in LPS-induced ALI is mediated by JNK and p38 MAPK pathway. (a) Western blot detected the expression of ERK, JNK, and p38 MAPKs and their phosphorylated protein in lung tissue after LPS-induced injury. (b) Western blot detected cytochrome c and caspase-3 expression after inhibition of UCP2-induced activation of MAPK signaling pathways by the inhibitors of specific MAPK signaling pathways, respectively. (c-d) Quantification of cytochrome c and caspase-3 immunoreactivity using Western blot analysis. (e) Quantification of the phosphorylated protein p-ERK, p-JNK, and p-p38 shown in graphs using densitometric analysis. (f) The levels of TNF-α in BALF were measured by ELISA. (g) The levels of IL-1β in BALF were measured by ELISA. (h) The levels of ROS in lung were detected by MDA assay ( versus NC group, versus LPS group, and versus UCP2-Ad + LPS group). Values are presented as means ± SEM ().

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)