IL-33 Effect on Quantitative Changes of CD4⁺CD25^highFOXP3⁺ Regulatory T Cells in Children with Type 1 Diabetes

Figure 1

Representative flow cytometric analysis of T cells in DM1 patient after in vitro IL-33 treatment. PBMCs from diabetic type 1 patient were cultured and stimulated as described in Material and Methods, stained with antibodies against Treg associated molecules, and analyzed using flow cytometry. Analyzing T cells, dot plots representing anti-CD4 versus SS were carried out to establish CD4⁺ and CD4⁻ lymphocyte gates. Then, the following dot plots were generated: anti-CD4 versus CD127 from CD4⁺ gate. After gating on CD4⁺127⁻ cells, the frequency of nonstimulated (a) and IL-33 stimulated (b) cells was determined. Gated, nonstimulated (c) and IL-33 stimulated (d) T cells were checked for the expression of FOXP3 defined as Mean Fluorescence Intensity. Similarly, the expression of ST2 on nonstimulated (f) and IL-33 stimulated (g) was analyzed. During the analysis isotypes controls for FOXP3 (e) and ST2 (h) staining were used. MFI: Mean Fluorescence Intensity.

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