Glutathione Peroxidase-1 Suppresses the Unfolded Protein Response upon Cigarette Smoke Exposure

Figure 4

RSV infection enhances the UPR in the lung. (a) GPx-1 and CHOP gene expression were determined in NHBE cells isolated from nonsmoker (NS), smokers (SM), and COPD (COPD) individuals ( donors per group) infected with mock or RSV and analyzed by qPCR. (b) Protein expression of GPx-1, ATF6, ATF4, CHOP, and β-actin was examined by immunoblots. (c) Wild type mice were exposed to cigarette smoke or room air for six months and subsequently infected with 1 × 10⁶ pfu of RSV. Animals were euthanized at 0, 1, 3, 5, 7, and 9 days after infection (dpi) and Gpx-1 and Chop expression were determined by qPCR. (d) Protein expression of GPx-1, ATF6, ATF4, CHOP, and β-actin was examined by immunoblots. Data are shown as mean ± SEM, where each measurement was performed on 3 independent days. denotes a value < 0.05, when comparing both treatments connected by a line or the same infection day, determined by Student’s -test (2 groups) or 2-way ANOVA with Tukey’s post hoc test (>2 groups).

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