Glutathione Peroxidase-1 Suppresses the Unfolded Protein Response upon Cigarette Smoke Exposure

Figure 5

Gpx-1 deficient mice have heightened ER stress in their lungs following exposure to cigarette smoke. Gpx-1^−/− and wild type mice were exposed to cigarette smoke daily for 1 year. (a) Lung gene expression of Chop, Atf4, Edem1, Grp78, Grp94, and Xbp1 was examined. (b) Immunoblots were performed of whole lung protein for CHOP, ATF4, EDEM, GRP78, GRP94, and XBP1. Dot plots are represented as (a) relative quantification (RQ) compared to ACTB expression or (b) densitometry units (DU) of pixel intensity expressed as a ratio to β-actin. Every lane represents an individual mouse. Data are shown as mean ± SEM, where each measurement was performed on 3 independent days on 6 donors/group. denotes a value < 0.05, when comparing both treatments connected by a line, determined by 2-way ANOVA with Tukey’s post hoc test (>2 groups).

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