Arginine Relieves the Inflammatory Response and Enhances the Casein Expression in Bovine Mammary Epithelial Cells Induced by Lipopolysaccharide

Figure 3

Effects of Arg on iNOS expression in LPS-treated BMECs. Pure 2nd generation BMECs were starved for 16 h and then cultured for 12 h in DMEM/F12 medium containing 0 or 10 μg/mL LPS and 0 or 100 μg/mL Arg. The mRNA levels of iNOS were analyzed using qPCR (a). β-Actin was used as an internal reference. 0.5 mL cellular supernatants were gathered at 1 h and 12 h separately after cells were incubated with challenge medium. Then NO content in supernatants was analyzed using NO detection kit (b). Data shown are mean ± SEM of three independent experiments. Means with different letters (A, B, or C) are significantly different () from each other.

(a)

(b)