Arginine Relieves the Inflammatory Response and Enhances the Casein Expression in Bovine Mammary Epithelial Cells Induced by Lipopolysaccharide

Table 1

Primers utilized for quantitative real-time PCR analysis.

Gene	Forward primer	Reverse primer	Product; size (bp)	Amplification; efficiency
β-Actin	CCTGCGGCATTCACGAAACTAC	ACTCCTGCTTGCTGATCCACATC	273	96.75%
TLR-4	CGGCACAGACAGAGGGTTAT	GGTCCAGCATCTTGGTTGAT	240	104.15%
NF-κB	CCCTTCCAAGTTCCCATAGAA	CTCCCAGAGTTCCGATTCAC	197	93.55%
iNOS	GGACTTGGCTACGGAACTGG	GGTGAAGCGTGTCTTGGAAA	257	89.21%
IL-1β	CCTCCGACGAGTTTCTGTGT	AAAGCTCATGCAGAACACCA	161	93.51%
IL-6	GCTCTCATTAAGCGCATGGT	AGCAAATCGCCTGATTGAAC	172	95.38%
TNF-α	CCTGCCAATGTTTCCAGACT	GGCAGCCCTTAGTTTGTGTC	183	99.71%
PI3K	GATGCTACCTTACGGCTGCT	CGGCACAGGATAGGGTAAAC	215	94.39%
AKT	CACCATTACGCCACCTGAC	CACTCAAACGCATCCAGAAA	233	101.27%
mTOR	CATGTGCGAACACAGCAACA	TGCCTTTCACGTTCCTCTCC	149	94.59%