Research Article
Proliferative Effects of Histamine on Primary Human Pterygium Fibroblasts
Figure 4
The expression and role of histamine receptors on HPFs. (a) DNA agarose gel electrophoresis showed the expression of H1R, H2R, and H4R on HPFs. (b) RT-qPCR showed that the expression of H1R was about 5-fold more than H2R and the expression of H4R was quite few. (c) MTT assay showed that H1R antagonist significantly inhibited the proliferation of HPFs induced by histamine. (d) MTT assay showed that H2R antagonist did not show the inhibition effect. compared to incubation with 10 μmol/L of histamine alone. compared to incubation without histamine and antagonist.
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