Antitumor Macrophage Response to Bacillus pumilus Ribonuclease (Binase)

Figure 7

Influence of binase treatment on macrophage polarization markers and proinflammatory mediators in THP-1-derived macrophages. THP-1 monocytes were differentiated in the presence of PMA-conditioned medium and exposed to medium only (control, dotted line) or to RNA (10 μg/ml), RNase1 (10 μg/ml), binase (10 μg/ml), LPS (100 ng/ml), or IL-4 (10 ng/ml) for 0.5 h as indicated. In all cases, mRNA expression of TNF-α, IL-6, IL-12, iNos, Cd206, and Ym1/2 was analyzed by real-time PCR. Data are expressed as changes in the ratio between target gene and β-actin mRNA expression. The results were obtained from six independent experiments carried out in duplicates. Values represent mean ± SD. versus control, ns = nonsignificant.