Administration of FTY720 during Tourniquet-Induced Limb Ischemia Reperfusion Injury Attenuates Systemic Inflammation

Figure 3

Change in the distribution of T cell subsets in the peripheral blood and spleen following tourniquet-induced hind limb IRI. Rats underwent hind limb ischemia by placement of a tourniquet band for 4 hr followed by reperfusion of affected limb. Rats received a single intravenous bolus of either vehicle control (•) or 0.3 mg/kg FTY720 (■) 15 min after placement of the tourniquet. Animals were euthanized at 6, 24, or 72 hr postinjury as indicated. Whole blood and isolated splenocytes were stained for expression of T cell markers CD4, CD8, αβ, and Foxp3. Using 3-color flow cytometry, gated CD4⁺αβ⁺Foxp3⁻, CD4⁺αβ⁺Foxp3⁺, and CD8⁺αβ⁺ lymphocytes were enumerated. Data are presented as the calculated mean ± SEM number of circulating cells per mL of blood and per spleen (n = 4–10 rats per time point/group). compared with vehicle control at the same time point postinjury.

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