Administration of FTY720 during Tourniquet-Induced Limb Ischemia Reperfusion Injury Attenuates Systemic Inflammation

Figure 4

Proinflammatory cytokine and chemokine in the serum following tourniquet-induced hind limb IRI. Rats underwent hind limb ischemia by placement of a tourniquet band for 4 hr followed by reperfusion of affected limb. Rats received a single intravenous bolus of either vehicle control (•) or 0.3 mg/kg FTY720 (■) 15 min after placement of the tourniquet. Animals were euthanized at 6, 24, or 72 hr postinjury as indicated. Collected serum was assayed in duplicate for levels of circulating G-CSF, GM-CSF, MIP-1α, IL-1β, IL-6, IL-10, IL-12p70, IFN-γ, IL-17α, IL-18, MCP-1, IP-10, MIP-2, TNFα, and RANTES using a multiplex bead assay. Serum from naïve control animals was used for baseline measurements (time 0). Data are shown as the mean serum concentration (pg/mL) ± SEM (n = 7–10 rats per time point/group). compared with vehicle control at the same time point postinjury.