Administration of FTY720 during Tourniquet-Induced Limb Ischemia Reperfusion Injury Attenuates Systemic Inflammation

<div>The number of lung infiltrating MPO<sup>+</sup> leukocytes was significantly increased in FTY720-treated rats following tourniquet-induced hind limb IRI. Formalin fixed-paraffin embedded lung tissue was stained at 6, 24, and 72 hr for polymorphonuclear neutrophils infiltrating the lung as determined by neutrophil-specific MPO IHC staining. Digital brightfield images were taken at 200x total magnification and analyzed using Image-Pro Plus software (v7.0, Media Cybernetics). A total of 10 fields per slide/animal were analyzed. Data presented as the mean ± SEM number of MPO<sup>+</sup> leukocytes per field. Vehicle control (•, <svg height="8.32035pt" id="M22" style="vertical-align:-0.2063999pt" version="1.1" viewbox="-0.0498162 -8.11395 27.8368 8.32035" width="27.8368pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M495 86L479 114C446 82 419 66 409 66C401 66 401 72 406 97C420 166 436 231 453 297C489 435 454 448 428 448C406 448 384 439 354 422C305 394 222 327 161 247H159L183 345C200 415 194 448 173 448C143 448 82 410 23 351L38 325C64 349 95 371 105 371C111 371 116 365 109 336L25 -4L31 -12C50 -4 77 3 107 9C119 69 132 122 145 168C197 254 321 381 370 381C387 381 393 374 378 305L329 95C309 17 320 -12 345 -12C372 -12 430 19 495 86Z" id="g113-111"></path></g><g transform="matrix(.013,0,0,-0.013,10.158,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z" id="g117-34"></path></g><g transform="matrix(.013,0,0,-0.013,21.421,0)"><path d="M153 550H386L412 615L406 623H120L82 318C104 327 142 338 184 338C294 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Mediators of Inflammation

fig5

Figure 5

Figure 5: Administration of FTY720 during Tourniquet-Induced Limb Ischemia Reperfusion Injury Attenuates Systemic Inflammation 