Research Article

Administration of FTY720 during Tourniquet-Induced Limb Ischemia Reperfusion Injury Attenuates Systemic Inflammation

Figure 5

The number of lung infiltrating MPO+ leukocytes was significantly increased in FTY720-treated rats following tourniquet-induced hind limb IRI. Formalin fixed-paraffin embedded lung tissue was stained at 6, 24, and 72 hr for polymorphonuclear neutrophils infiltrating the lung as determined by neutrophil-specific MPO IHC staining. Digital brightfield images were taken at 200x total magnification and analyzed using Image-Pro Plus software (v7.0, Media Cybernetics). A total of 10 fields per slide/animal were analyzed. Data presented as the mean ± SEM number of MPO+ leukocytes per field. Vehicle control (•, rats) and FTY720-treated (■, rats) at the indicated time points postinjury. compared with vehicle control at the same time point postinjury.