Research Article
Administration of FTY720 during Tourniquet-Induced Limb Ischemia Reperfusion Injury Attenuates Systemic Inflammation
Figure 7
Differential expression of selected genes in ischemic muscle, liver, and lung tissue in FTY720-treated rats (■) versus vehicle control-treated rats (•) following tourniquet-induced hind limb ischemia. Tissues at the indicated time point post-IRI were stored in RNALater and subsequently processed for mRNA. cDNA conversion of 1 μg of RNA was performed by RT-PCR followed by semiquantitative real-time PCR for gene expression analysis using the 2−ΔΔCT method. A custom low-density array panel of 88 genes relevant to ischemia-reperfusion injury was selected for the analysis. For each tissue, gene expression was quantified relative to naïve control animals. compared with vehicle control at the same time point postinjury.