Research Article

Administration of FTY720 during Tourniquet-Induced Limb Ischemia Reperfusion Injury Attenuates Systemic Inflammation

Table 3

Grading scheme for focal and multifocal skeletal muscle lesions.

SeverityPercent of skeletal muscle section affectedGradeQuantifiable finding

Normal00No foci
Minimal<5%11-2 foci
Mild5–25%23–6 foci
Moderate25–50%37–12 foci
Marked50–75%4>12 foci
Severe75–100%5Diffuse

Scoring methodology: 1: assess skeletal muscle at 200x magnification; 2: for every field, estimate the percent of the visual field containing necrotic myocytes (0%, 25%, 50%, 75%, or 100%); 3: average 10 fields; 4: convert the percentage to a grade based on the grading scheme.