Administration of FTY720 during Tourniquet-Induced Limb Ischemia Reperfusion Injury Attenuates Systemic Inflammation

Table 3

Grading scheme for focal and multifocal skeletal muscle lesions.

Severity	Percent of skeletal muscle section affected	Grade	Quantifiable finding
Normal	0	0	No foci
Minimal	<5%	1	1-2 foci
Mild	5–25%	2	3–6 foci
Moderate	25–50%	3	7–12 foci
Marked	50–75%	4	>12 foci
Severe	75–100%	5	Diffuse

Scoring methodology: 1: assess skeletal muscle at 200x magnification; 2: for every field, estimate the percent of the visual field containing necrotic myocytes (0%, 25%, 50%, 75%, or 100%); 3: average 10 fields; 4: convert the percentage to a grade based on the grading scheme.