IL-1β-Induced Accumulation of Amyloid: Macroautophagy in Skeletal Muscle Depends on ERK

Figure 1

IL-1β and IFN-γ induced autophagy. (a) Immunofluorescence analysis revealed IL-1β and IFN-γ induced accumulation of autophagosomes in GFP-LC3-transfected rhabdomyosarcoma cells. Inhibition of autophagy was exerted by 3-MA treatment. Quantitative greyscale analysis of LC3 fluorescence intensity of cells incubated with IL-1β and IFN-γ was carried out using Image J software. Results are calculated as mean of three independent experiments and statistical significance is indicated by . (b) Western blotting: autophagosome-associated LC3-II and free cytosolic LC3-I were distinguished by molecular weight (16 and 18 kDa, resp.). 48 hours exposure of human rhabdomyosarcoma cells to IL-1β and IFN-γ led to increased levels of LC3-II. One of three independent experiments with identical results is shown. Greyscale analysis of the LC3-II signal was carried out using Image J software and results are shown as mean plus standard error from three independent experiments. Statistical significance is indicated by .

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