IL-1β-Induced Accumulation of Amyloid: Macroautophagy in Skeletal Muscle Depends on ERK

Figure 3

IL-1β and IFN-γ effects are mediated via ERK signalling pathway. Immunoblotting analysis of human muscle cells (CCL136) exposed to IL-1β and IFN-γ and simultaneously treated with increasing concentrations of the p42/44 (ERK) MAP kinase inhibitor PD98059 for 24 hours. IL-1β and IFN-γ led to increased phosphorylation of ERK. Treatment with the ERK inhibitor PD98059 resulted in a trend towards decreased protein levels of CXCL-9. One of two experiments with identical results is shown.