Platelet-Released Growth Factors Induce Differentiation of Primary Keratinocytes

Figure 1

PRGF dose dependently influences the expression of differentiation markers in primary keratinocytes. PRGF stimulation of primary keratinocytes over 24 hours caused a dose-dependent decrease of keratin 1 (a) and keratin 10 (b) gene expression paralleled by a dose-dependent increase of transglutaminase-1 (c) and involucrin (d) gene expression (real-time PCR analysis, , , , ns = not significant, one-way ANOVA with Tukey’s multiple comparison test).

(a)

(b)

(c)

(d)