Platelet-Released Growth Factors Induce Differentiation of Primary Keratinocytes

Figure 4

The epidermal growth factor receptor (EGFR) partially mediated the keratin 1, keratin 10, transglutaminase-1, and involucrin gene expression in keratinocytes stimulated with PRGF. PRGF stimulation of primary human keratinocytes caused a reduced keratin 1 and keratin 10 gene expression and an induced transglutaminase-1 and involucrin gene expression. Costimulation with the EGFR-blocking antibody cetuximab revealed that these effects were mediated via the EGFR (real-time PCR analysis, , , , one-way ANOVA with Tukey’s multiple comparison test).

(a)

(b)

(c)

(d)