Andrographolide Activates Keap1/Nrf2/ARE/HO-1 Pathway in HT22 Cells and Suppresses Microglial Activation by Aβ₄₂ through Nrf2-Related Inflammatory Response

Figure 3

Effect of andrographolide on ARE gene transcriptional activity and HO-1 expressions. The abbreviation of andrographolide, ANDRO, was used. (a) HT22-ARE cells were treated with 100 nM brusatol for 2 h prior to andrographolide treatment at 1, 5, or 10 μM concentrations for 16 h. The cells were lysed, and the lysates were used to determine luciferase activity. The data are presented as the means ± SE of triplicates. The significance presented as compared with control group, compared with the brusatol-untreated group. (b) Relative mRNA expression levels of Nrf2 were regulated after andrographolide treatment. HT22 cells were seeded onto 12-well plates at 1.5 × 10⁵ cells per a well and treated with 1, 5, and 10 μM andrographolide for 24 h. The mRNA was extracted, cDNA was synthesized, and the relative expression levels of mRNA were determined. (c) HO-1 expression after andrographolide treatment at 1, 5, or 10 μM concentrations for 24 h in HT22 cells was determined by Western blot analysis. The data are presented as the means SE of triplicates. The significance presented as compared with control group.

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