Andrographolide Activates Keap1/Nrf2/ARE/HO-1 Pathway in HT22 Cells and Suppresses Microglial Activation by Aβ₄₂ through Nrf2-Related Inflammatory Response

Figure 5

Effect of andrographolide on pNF-κB or inflammatory protein expression in BV-2 cells. BV-2 cells were seeded and transfected with Aβ₄₂ plasmids for 12 h, and the medium was then replaced with fresh phenol red free medium containing 1, 5, or 10 μM of andrographolide for 24 h. The abbreviation of andrographolide, ANDRO, was used. (a) Nuclear extracts were used to determine pNF-B (Ser536) accumulation in the nucleus. Lamin B was used as a positive control. The data are presented as the means SE of triplicates. The significance presented as compared with control group and compared with the Aβ₄₂-transfected group. (b) Whole cell lysates were used to analyze i-NOS expression, determined by Western blot analysis. The data are presented as the means SE of triplicates. The significance presented as compared with control group and compared with the Aβ₄₂-transfected group. (c) COX-II expression was analyzed by Western blot analysis. The data are presented as the means SE of triplicates. The significance presented as compared with control group and compared with the Aβ₄₂-transfected group.

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