MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression

Figure 2

The severe asthma mice were mediated by Th17 cells. (a) Lung tissues were stained for immunohistochemistry (anti-IL-17, anti-IL-4) of the three groups. Histological analyses of lungs from the severe group exhibited markedly enhanced cells with stained IL-17 positively, but cells with stained IL-4 positively were not obviously different compared with those from the conventional group. (b) Western blot analysis detected IL-17 and IL-4 protein expression in the three groups. The IL-17 protein expression of the lungs from the severe group was higher than that from the conventional group, whereas IL-4 protein expression values were not obviously different between them. (c) Th17 and Th2 cells were tested in bronchial lung tissue suspension cells and splenocytes, and the cells were then subjected to intracellular staining of APC-anti-IL-17 and PE-anti-IL-4 by flow cytometry analyses. Th17 cells were the most in bronchial lung tissue suspension cells and splenocytes from the severe group compared with the conventional group, whereas Th2 cells were not obviously different between them.

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