MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression

Figure 7

IL-17 expression and Th17 cell differentiation under joint MBD2 and IRF4 gene silencing or overexpression. (a) Under joint M(−)/I(−), IL-17 protein expression was significantly the lowest according to Western blot analyses; under joint M(−)/I(0), IL-17 protein expression was higher than M(−)/I(−). While under joint M(+)/I(+), IL-17 protein expression was significantly the highest; under joint M(+)/I(0), IL-17 protein expression was lower than M(+)/I(+). IL-17 protein expression showed no significant difference between the control group, M(0)/I(0), M(−)/I(+), and M(+)/I(−). (b) Under joint M(−)/I(−), Th17 cell differentiation was significantly the lowest according to flow cytometry analyses; under joint M(−)/I(0), Th17 cell differentiation was higher than M(−)/I(−). Under joint M(+)/I(+), Th17 cell differentiation was significantly the highest; under jointM(+)/I(0), Th17 cell differentiation was lower than M(+)/I(+). No significant difference in Th17 cell differentiation was observed between the control group, M(0)/I(0), M(−)/I(+), and M(+)/I(−).

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