The Antimalarial Chloroquine Suppresses LPS-Induced NLRP3 Inflammasome Activation and Confers Protection against Murine Endotoxic Shock

Figure 1

CQ suppress IL-1β and IL-18 secretion in LPS- and ATP-primed BMDMs. BMDMs were pretreated with or without 0.4, 4, and 40 μM CQ for 1 h and then stimulated with LPS (100 ng/ml) for 4 h and ATP (2 mM) for an additional 1 h. The IL-1β and IL-18 levels in culture supernatants were determined by ELISA (). versus LPS plus ATP group (a-b). LDH release from BMDMs was measured by LDH assay (c). , versus LPS plus ATP group. All data were expressed as mean ± SEM in triplicate and are representative of three independent experiments.

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