The Antimalarial Chloroquine Suppresses LPS-Induced NLRP3 Inflammasome Activation and Confers Protection against Murine Endotoxic Shock

Figure 2

CQ inhibits transcription and maturation of IL-1β and IL-18. (a) BMDMs were pretreated with CQ (4, 40 μM) for 1 h, primed with LPS (100 ng/ml) for 4 h, and stimulated with ATP (2 mM) for an additional 1 h. Cell lysates and supernatants were prepared and IL-1β level was detected by ELISA. versus LPS plus ATP group. (b) IL-1β and IL-18 mRNA expressions were measured by qRT-PCR. BMDMs were pretreated with CQ (40 μM) for 1 h and then primed with LPS (100 ng/ml) for 2 h. Data are shown as the means ± SEM (). versus LPS group; versus LPS plus ATP group.

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