The Antimalarial Chloroquine Suppresses LPS-Induced NLRP3 Inflammasome Activation and Confers Protection against Murine Endotoxic Shock

Figure 4

CQ dampens NLRP3 inflammasome activation. BMDMs were pretreated with CQ (4, 40 μM) for 1 h and then stimulated with LPS (100 ng/ml) for 4 h and ATP (2 mM) for an additional 1 h. (a) Nlrp3 mRNA expression was measured by qRT-PCR. (b) NLRP3 and GAPDH were measured in cell lysates by western blot. (c) Western blot analysis of cleaved caspase-1 (p10) in culture supernatants (Sup) or of procaspase-1, ASC, and tubulin in cell lysates (Lys). (d) BMDMs were pretreated with CQ at the indicated concentration for 1 h and then stimulated with LPS (100 ng/ml) for 4 h and nigericin (10 μM) for 30 min. Production of mature IL-1β in supernatants was measured by ELISA. Data are shown as means ± SEM; , versus LPS plus ATP group.

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