The Antimalarial Chloroquine Suppresses LPS-Induced NLRP3 Inflammasome Activation and Confers Protection against Murine Endotoxic Shock

Figure 6

CQ protects mice against lethal endotoxic shock by suppressing NLRP3 inflammasome activation. CQ improved survival in endotoxic shock murine model. BALB/c murine mice ( per group) were injected with a lethal dose of LPS (15 mg/kg) alone or together with CQ (10, 30 mg/kg). (a) Survival in each group was observed for 168 h and the log-rank test was used for intergroup comparison. versus LPS group. (b) IL-18 and IL-1β levels in serum at 6 h were measured by ELISA. (c) IL-18 and IL-1β levels in lung homogenates at 6 h were measured by ELISA ( per group). (d) IL-18 and IL-1β levels in peritoneal lavage fluid at 6 h were measured by ELISA ( per group). Data are shown as means ± SEM. , versus LPS group. (e) Caspase-1 activation and NLRP3 expression in the lung were detected by western blot ().

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