Anti-Inflammatory Activity of Crude Venom Isolated from Parasitoid Wasp, Bracon hebetor Say

Figure 4

Reduction of serum TNF-α and MDA levels by BHV. For the chronic study of septic shock in mice by LPS, mice were treated with venom for 5 days and then were given the LPS injection. 3 days later, they were euthanized and blood and tissues were collected for TNF-α and MDA levels by commercially available kits. TNF-α levels (a) and MDA levels (b). Values in bar graphs are mean ± SEM of three independent experiments. is considered significant compared to LPS group only. For hematoxylin and eosin staining, lung and testis tissues were stored in 10% neutral buffered formalin (NBF) and then stained according to standardized protocol for (H&E) staining. Lungs (c–e) and testis (f–h). Scale bar 100 μm.

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