A Novel CD48-Based Analysis of Sepsis-Induced Mouse Myeloid-Derived Suppressor Cell Compartments

Figure 1

Purity of granulocytic and monocytic subpopulations identified by Ly6G and Ly6C. (a) Gating of monocytic and granulocytic MDSCs by Ly6C and Ly6G expression level. Cells were obtained from bone marrow, spleen, and blood from naïve and 7-day CLP Gfi1:GFP knock-in mice. MDSCs were gated out from CD11b⁺ cells and sorted as granulocytes and monocytes. (b) Representative photomicrographs (images of bone marrow are shown) of Wright–Giemsa stained MDSC subsets; 200 cells were counted in each cell preparation. Bar = 10 μm, monocyte, segmented granulocyte, ring granulocyte. (c) Analysis of MDSC subset purity by cell morphology. (d) Gfi1:GFP fluorescence of each MDSC subset was read. Representative graphs of flow cytometry analysis are shown. (e) Purity analysis of MDSC subsets by FACS. Data are mean ± SEM of 5–8 mice per group, , , , .

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