UDP-Induced Phagocytosis and ATP-Stimulated Chemotactic Migration Are Impaired in STIM1^−/− Microglia In Vitro and In Vivo

Figure 1

Lipopolysaccharide-induced phagocytosis and cytokine secretion in wild-type and STIM1^−/− microglia. (a, b) Representative images of image-based phagocytosis measurement using phagocytic engulfment of FITC bead in WT and STIM1^−/− microglia. Summary of five independent phagocytosis measurements. Seven images were used for phagocytosis measurements and then depicted as a bar graph. in LPS-induced fold-induction of WT; in WT mock, . (c, d) Comparisons of LPS-induced cytokine secretion activity: TNF-α secretion (c) and IL-6 secretion (d) were compared in WT and STIM1^−/− microglia. , , where is independent ELISA experiment; ns, no significant difference from WT mock.

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