UDP-Induced Phagocytosis and ATP-Stimulated Chemotactic Migration Are Impaired in STIM1^−/− Microglia In Vitro and In Vivo

Figure 2

Comparison of UDP-induced Ca²⁺ signalling and phagocytosis in wild-type and STIM1^−/− microglia. (a) Representative curves of UDP-induced mobilization in WT and STIM1^−/− microglia; 100 μM UDP induced ER-Ca²⁺ efflux, whilst store depletion induced Ca²⁺ influx. (b) Relative UDP-induced Ca²⁺ transient release from ER. , . (c) Comparison of UDP-induced SOCE activity determined by the slope of Ca²⁺ influx. , . (d) Representative flow cytometry images of the phagocytosis measurements. FITC intensities of phagocytic bead engulfment with or without 100 μM UDP were measured at 20 min. Grey region indicates the population of bead-engulfed microglia. (e) Summary of FACS from three independent phagocytosis experiments. Geometric mean represents the relative phagocytosis activity in each set. in comparisons of bead engulfment in WT microglia.

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