Anti-Inflammatory and Neuroprotective Role of Natural Product Securinine in Activated Glial Cells: Implications for Parkinson’s Disease

Figure 3

Securinine represses LPS-induced proinflammatory cytokines and iNOS expression in BV2 and mouse primary microglia. (a) Securinine inhibits proinflammatory cytokine expression following LPS exposure. BV2 and primary microglial cells were preincubated with securinine for 1 h before stimulation with LPS (500 ng/mL). After 6 h of stimulation, total RNA was isolated and levels of mRNA expression of proinflammatory cytokines TNF-α and IL-1β analyzed via quantitative real-time PCR ((A1) BV2 cells and (A2) mouse primary microglia). (b) Securinine represses LPS-induced iNOS mRNA and protein expression in BV2 (B1) and primary microglia cells. After 24 h of stimulation with LPS (500 ng/mL), the level of iNOS protein was monitored by Western blot analysis ((B1) BV2 cells and (B2) primary microglia cells). Data are mean ± SD of three different experiments. versus control; versus LPS-treated group alone.

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