Toll-Like Receptor 9 Promotes Survival in SERCA2a KO Heart Failure Mice

Figure 5

Picrosirius staining and hydroxyproline analysis demonstrated a trend towards increased collagen deposition in both HF groups. (a) Representative picrosirius red-stained cardiac sections. The size marker is 50 μM. (b) Image-based quantification of picrosirius red staining (WT, ; TLR9 KO, ; SERCA2a KO, ; and SERCA2a/TLR9 KO, ). (c) Hydroxyproline (μg/ml dry weight) measurements of collagen (WT, ; TLR9 KO, ; SERCA2a KO, ; and SERCA2a/TLR9 KO, ). (d, e) Left ventricular gene expression (WT, ; TLR9 KO, ; SERCA2a KO, ; and SERCA2a/TLR9 KO, ) of collagen I mRNA (Col I, d) and collagen III mRNA (Col III, e) determined by quantitative RT-PCR and presented relative to expression of GAPDH mRNA. The groups were compared using two-way ANOVA with Tukey’s multiple comparison test post hoc. Data are mean ± SEM. versus corresponding control.

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