Chemopreventive Effects of Silibinin on Colitis-Associated Tumorigenesis by Inhibiting IL-6/STAT3 Signaling Pathway

Figure 4

Silibinin supplementation inhibited proliferation and promoted apoptosis in intestinal tumor cells. (a, b) IMCE and HCT-116 cells were treated with silibinin at indicated concentrations for 72 h, respectively. Cell viability was then determined by MTT assay. Data are representative of three independent experiments and expressed as mean ± SD. (c, d) Colon sections from the AOM/DSS and AOM/DSS/SB groups were stained with Ki-67 (brown staining). (e, f) Colon sections from the two groups were stained with TUNEL. Green staining represented apoptotic cells. Scale bars, 50 μm. Positive rate was determined by counting positively stained nuclei in tumor cells at 5 randomly selected fields from each group. SB: silibinin. (, , AOM/DSS vs. AOM/DSS/SB).

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