Chemopreventive Effects of Silibinin on Colitis-Associated Tumorigenesis by Inhibiting IL-6/STAT3 Signaling Pathway

Figure 5

Silibinin supplementation restored colonic barrier function and ameliorated the infiltration of macrophages. (a) Paraffin-embedded specimens of colonic tumor-adjacent tissue were subjected to immunofluorescent staining for ZO-1 distribution using an anti-ZO-1 antibody and a FITC-labeled secondary antibody (green staining). Nuclei were stained with DAPI (blue staining). (b) Real-time PCR analysis of ZO-1 expression in colonic epithelium was also shown. (c, d) PAS staining for goblet cells in tumor-adjacent tissue was shown, and diagrams presented average positive cells per crypt of each group. (e, f) Expression of F4/80 in tumor-adjacent tissue was assessed using immunohistochemical staining. The positive rate was determined by counting the absolute number of positive staining in at least 100 colonic crypts of each mouse. Scale bars, 50 μm. SB: silibinin. (, , AOM/DSS vs. AOM/DSS/SB).

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