Chemopreventive Effects of Silibinin on Colitis-Associated Tumorigenesis by Inhibiting IL-6/STAT3 Signaling Pathway

Figure 6

Silibinin inhibited STAT3 phosphorylation in intestinal tumor cells. (a) Western blot showed the expression of p-STAT3 and STAT3 in IMCE cells after incubation with silibinin and/or LPS for 3 h, 6 h, 12 h, and 24 h, respectively. (b) ImageJ was applied to densitometric analyses to determine the expression of p-STAT3 and STAT3 in IMCE cells, and the data were normalized as relative ratio to β-actin. (c) The expression of p-STAT3 and STAT3 in HCT-116 cells after incubation with silibinin and/or LPS for 3 h, 6 h, 12 h, and 24 h, respectively. (d) Densitometric analyses to determine the expression of p-STAT3 and STAT3 in HCT-116 cells. (e) Western blot was also utilized to show the expression of p-STAT3 and STAT3 in the control, AOM/DSS, and AOM/DSS/SB groups. (f) Densitometric analyses were also involved in quantifying the expression of p-STAT3 and STAT3 in the control, AOM/DSS, and AOM/DSS/SB groups. SB: silibinin; ns: not significant. (, , AOM/DSS vs. AOM/DSS/SB).

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